• strict warning: Non-static method view::load() should not be called statically in /var/www/html/sites/all/modules/views/views.module on line 906.
  • strict warning: Declaration of views_handler_filter::options_validate() should be compatible with views_handler::options_validate($form, &$form_state) in /var/www/html/sites/all/modules/views/handlers/views_handler_filter.inc on line 607.
  • strict warning: Declaration of views_handler_filter::options_submit() should be compatible with views_handler::options_submit($form, &$form_state) in /var/www/html/sites/all/modules/views/handlers/views_handler_filter.inc on line 607.
  • strict warning: Declaration of views_rotator_style_plugin::options() should be compatible with views_object::options() in /var/www/html/sites/all/modules/views_rotator/views_rotator_style_plugin.inc on line 56.
  • strict warning: Declaration of views_plugin_row::options_validate() should be compatible with views_plugin::options_validate(&$form, &$form_state) in /var/www/html/sites/all/modules/views/plugins/views_plugin_row.inc on line 134.
  • strict warning: Declaration of views_plugin_row::options_submit() should be compatible with views_plugin::options_submit(&$form, &$form_state) in /var/www/html/sites/all/modules/views/plugins/views_plugin_row.inc on line 134.

Cultivation Technology of Shiitake Mushroom (Lentinula edodes)

Cultivation technology of Shiitake mushroom (Lentinula edodes)


The cultivation technology of the edible and medicinal mushroom Lentinula edodes commonly called as Shiitake mushroom has been standardized. The flow chart of the technology is as follows.


Substrate preparation

(Mixture of hard wood sawdust, wood chips, Rice bran and calcium sulphate)



Fill one Kg wet substrate (65% moisture) to Polypropylene bags, plug with non-absorbent cotton



Sterilize the substrate (121°C, 15 lb pressure for 90-120 minutes in autoclave)



Cool the bags to room temperature


Spawning (Aseptically, @ 5% of wet substrate)


Spawn running (22-26°C, 60-70 days)

Initial spawn running





Mycelial coat formation



Blister formation



Open bags for browning





Cold water shock treatment (10-15°C water, 6-8 hours)



Fructification (22-26°C, 80-85% R.H, Light, cross ventilation)


Pinheads                                 Ready to harvest mushrooms



Sold as fresh or dry mushroom


Services offered by the mushroom lab, IIHR



  1. Spawn of commercial varieties of Oyster mushroom (Pleurotus species), Milky mushroom (Calocybe indica), Shirotamogitake (Hypsizygous ulmarius) and Shiitake (Lentinula edodes) can be procured in person on prior order basis.


  1. Regular training courses are offered on Mushroom seed production and Mushroom cultivation technology separately.


  1. Literature – Mushroom cultivation Bulletin, folders on mushroom cultivation.




Further details: Director, IIHR, Hessaraghatta lake post, Hessaraghatta, Bangalore-560089, E-mail: director@iihr.ernet.in