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Technology for the establishment of pollen cryobank

 

Technology has been developed for long term cryopreservation of Nuclear Genetic diversity. Long term cryopreserved pollen in the form of nuclear genetic diversity (NGD) of citrus, papaya, grape, mango, tomato, eggplant, onion, capsicum, rose, gladiolus, gerbera, carnation and ret species of medicinal plants are maintained in liquid nitrogen. The pollen cryobank is managed by periodic replenishment of the cryogen, for maintaining a constant cryogenic temperature throughout the storage duration. This technology developed is globally acceptable and will be useful for production of hybrid seeds in the above-mentioned crops, besides its use in gene banks.

 

Although genetic conservation through pollen storage does not accomplish whole genome conservation, a breeder involved in genetic enhancement of a given crop can have access to a Pollen Cryobank facility, for nuclear genetic diversity (NGD) inputs in his amelioration program. Besides the already existing role of pollen cryobanks in breeding, there are many promising applications, which has come to focus with the recent advances in allied bio-scientific areas

 

Table 1:Pollen cryobank  species coverage

                                                                

Sl.No.

Crop

Species (Latin name)

No. of cultivars/accessions

1

 

Hemerocallis sp.

 

2

Mango

Mangifera indica

28

3

Citrus

Poncirus trifoliata

64

 

 

Citrus aurantifolia

 

 

C.limon

4

Carnation

Diathus caryophyllus

 

5

Gladiolus

Gladiolus species

20

 

 

G.callianthus

6

Grape

V.vinifera

81

 

 

V.labrusca

 

 

V.candicans

7

Papaya

Carica papaya

110

 

 

C.Cauliflora

 

 

C.goudotiana

8

Pomegranate

Punica granatum

10

9

Tomato

Lycopersicon esculentum

91

 

 

L.hirsutum

 

 

L,pimpinellifolium

 

 

L.peruvianum

10

Lemon

Citrus limon

7

 

 

C.aurantifolia

11

 

Catharanthus roseus

12

Onion

Allium cepa

27

 

 

A. fistulosum

13

Rose

Rosa sp.

40

 

 

R. indica

 

 

R. multiflora

 

14

Solanums

Solanum erianthum

91

 

 

S. indicum

 

 

S. insanum

 

 

S. integrifolium

 

 

S. macrocarpum

 

 

S. melongena

 

 

S. sisymbrifolium

 

 

S. torvum

 

 

S. viarum

 

THE POLLEN CRYOBANK AT IIHR, BANGLORE finds mention in Limca Book of Records-2001.

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