1. Protocols for large scale propagation of pointed gourd, hybrids of hippeastrum, tuberose and male sterile lines of cauliflower have been developed. Established embryogenic cultures from anthers of Capsisum for development of haploids. 2. Optimization of in vitro regeneration protocols for transgenosis was accomplished in tomato, chilli, onion and carnation. 3. The transgenic lines of tomato and chilli with chitinase gene have shown delayed and moderately reduced disease development as compared to control and preliminary studies with transgenic lines of tomato with PGIP gene have shown promise against late blight. Keeping this in view, pyramiding of tomato with multiple transgenes is being done using double or triple gene constructs for enhanced fungal resistance.
1. Mythili, J.B, Rajeev, P.R, Shailesh Yadav, Ramanjini Gowda, P.H, Girija Ganeshanand Chowdappa, P (2012). Stable integration and expression of chitinase gene in transgenic tomato . Acta Horticulturae 929: 373-379 2. Mythili, J.B, Rajeev, P.R and Chethana, B.S (2012). Factors influencing in vitro regeneration and Agrobacterium mediated transformation of onion cv Arka Niketan” Acta Horticulturae 929: 417-422 3. J B Mythili,G V S Saiprasad, C Naveena, P R Rajeev & K K Upreti (2011) Differential response of tomato and tobacco to Agrobacterium mediated transformation with cytokinin independent -1 ( CKI-1) gene as influenced by cytokinin levels. Indian Journal of experimental biology, 49: 909-918. 4. M. Kallesh Prasad, J.B. Mythili, Tejaswini, Lalitha Anand, H.J. Rashmi and C. Suneetha 2009. Optimization of regeneration protocol and Agrobacterium mediated transformation in carnation (Dianthus caryophyllus L.) J Hort sci. 4: 120-127 5. Saiprasad, GVS., Mythili, JB., Lalitha Anand, Suneetha, C, Rashmi, HJ, Naveena, C and Girija Ganeshan.(2008). Development of a Trtichoderma harzianum endochitinase gene construct conferring antifungal activity in transgenic tobacco. Indian Journal of Biotechnology . 8:199-206 6. Shivkumar,B, Mythili, J.B, Lalitha Anand and Saiprasad, G.V.S. 2007. Influence of genotype on Agrobacterium mediated transformation of tomato, Indian J Hort. 64 (3) 251-257 7. Mythili, J.B and Pious Thomas (2003) Effect of stress on induction of androgenic embryos in Capsicum annuum L. cv Arka Gaurav . J plant Biol. 30(3): 293-297 8. Shivegowda, ST, Mythili ,JB, Lalitha Anand, Saiprasad, GVS, Ramanjini Gowda and Gowda, TKS (2002) In vitro regeneration and transformation of chilli pepper (Capsicum annuum L.) J. Hort sci and Biotechnology, 77 (5)629-634. 9. Mythili, J. B. and Thomas, P. 1999. In vitro multiplication of pointed gourd (Trichosanthes dioica Roxb.) Scientia Horticulturae 79: 87-90. 10. Mythili, J. B. and Thomas, P. 1999. Influence of ammonium to nitrate nitrogen ratio in different basal media on callusing response of capsicum (Capsicum annuum L. grossum Sendt.) anthers, Indian Journal of Experimental Biology 37: 314-316.
Technology for development of micropropagation protocol in pointed gourd, tuberose, hippeastrum and male sterile lines of cauliflower was necessitated by the evergrowing demand and the need for disease free planting material. Further, the adoption of micropropagation technology is restricted by the non-availability of protocols to small scale entrepreneurs. The chitinase gene construct developed in house has been taken by few ICAR institutes and Universities
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