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Diagnostic kit to detect Citrus tristeza virus in citrus Dip stick format and ELISA format based on Monoclonal antibodies
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| Application / Use: | To detect CTV in citrus orchards | |
| Input Needed: | Bio-fermentor, Equipment for routine microbiology and protein isolation lab, BSc Biotechnology trained man power | |
| Output Capacity: | 1000 kits per day for medium sized production level | |
| Specific Benefits: | Planting disease free material is important in managing this disease. Certification of nursery material is very important by Govt agencies. This kit will be handy for use by semiskilled personnel for cheking the presence of the virus. The ELISA format is rapid and cost effective where upto 90 samples can be tested at one time. Secondly, early detection helps in removal and replanting of effected trees thereby preventing the spread of the disease. Orchards once affected continue to decline and die after about 5 years. | |
| Unit Cost: | Rs10/- once it is scaled-up for dipstick and Rs 100/ for ELISA plate (Needs scaling-up) | |
| Description: | The kit is simple to operate can be used by illiterate farmer in the field without any lab facilities as long as there is clean water. It involves crushing the leaf sample to get a few drops of sap which can be collected in a tube into which the stick coated with regents is dipped followed by dipping in detection regent and detection substrate. A positive and a negative control are included for reliability of the results. Washing of the dip stick in clean water are intermediary steps for which clean water can also be included in the kit. ELISA format is for the researcher and semi quantitative and quantitative estimations can be done | |
| Developed By: | Dr Akella Vani & PhD Students | |
| Contact Person: | Director, Indian Institute of Horticultural Research, Hessarghatta Lake, Bangalore-560 089, Tel: 080-28466420-24 (ext. 200); Fax: 080-28466291; email: director@iihr.ernet.in | |
| Institute: | IIHR, Bangalore | |