|
Diagnostic kit to detect Ralstonia solanacearum from infected tissues
|
||
| Application / Use: | To detect bacteria in wilt samples of more than 350 crop/plant species, It is specific to Ralstonia and does not cross react with Xanthomonas, Pseudomonas, and Erwinia | |
| Input Needed: | Bio-fermentor, Equipment for routine DNA isolation lab, BSc Biotechnology trained manpower | |
| Output Capacity: | 1000 kits per day for medium sized production level | |
| Specific Benefits: | Wilt is caused by a number of reasons. Early rouging of Ralstonia effected plants can prevent the disease from spreading through irrigation water. While at the same time wilt caused by other causes can be tackled accordingly. The field scientist is often unable to identify the causal organism to suggest remedial measures. This kit readily identifies Ralstonia from other pathogens and is sensitive enough to detect only 10 bacteria per ml of extract | |
| Unit Cost: | Rs10/- once it is scaled-up (Needs scaling-up) | |
| Description: | The kit is simple to operate can be used by illiterate farmers in the field as well as researchers without any lab facilities as long as there is clean water. It involves crushing the affected tissue in water in a tube into which reagents are added and after shaking well, the contents are allowed to settle. The top layer is transferred to another tube into which a stick coated with regents is dipped followed by dipping in detection regent and detection substrate. A positive and a negative control are included for reliability of the results. Washing of the dip stick in clean water are intermediary steps for which clean water can also be included in the kit. | |
| Developed By: | Dr Akella Vani & PhD Students | |
| Contact Person: | Director, Indian Institute of Horticultural Research, Hessarghatta Lake, Bangalore-560 089, Tel: 080-28466420-24 (ext. 200); Fax: 080-28466291; email: director@iihr.ernet.in | |
| Institute: | IIHR, Banglore | |