Brief summary of current areas of research: Presently am working on the three following aspects of molecular markers and the mining of molecular markers in next generation sequencing data. 1.Development of SCAR Markers: Here mainly focus on development of SCAR markers either from RAPDs or from SRAPs polymorphism. Presently 23 SCAR markers are developed in Ridge gourd related to Downey mildew resistance, 11 SCAR markers are developed in eggplant related to Ralostonia wilt resistance,6 SCAR markers are developed in citrus rootstocks validation and validated successfully and patent filing under process. 2.Validation of Available trait linked functional markers: Here, public available trait linked functional molecular markers used in main stream crop breeding programme. we are successful in validation of powdery mildew resistance linked SCAR /SSR markers in pea, mitochondrial gene specific primers linked to male sterility trait in carrot, Fusarium linked CAPs markers in watermelon and rust resistance molecular markers in French bean. 3. Construction of linkage maps Here , main focus on development of linkage maps to Ralstonia resistance wilt in eggplant, Fusarium wilt resistance in watermelon and mining of EST-SSR , Genomic SSR, SNPs from sequencing data set in most of the horticultural crops by using various bioinformatic tools
Lakshmana Reddy D C, Archana Bhardwaj, Radhika V and Aswath C 2012, “miRNAs in Solanum melongena–mined through In silico approach” Journal of Horticulture Science and Biotechnology, UK, Vol 87(2): 186-192 Lakshmana Reddy D C Srikanth H S, Aswath C and Singh T.H., 2012. SSR based DNA finger printing bar codes for eggplant varieties and hybrids. International Journal of vegetable science, 18:1-12 Taylor and Francis, UK D.C.Lakshmana Reddy, H .M. Mahadeva Swamy, R. Asokan, S.C.Venkatesh and T.K.S Gowda 2012., Isolation, Molecular characterization and diversity of Lepidopteron active cry type genes of Baillus thuringiensis isolates and their efficacy against Helicoverpa armigera Hubner( Noctuidae: Lepidoptera), Biopesticide International, Vol-8(2):93-106. Radhika V, Reddy DCL, Bhardwaj A, Aswath C 2011 In Silico mining of single nucleotide polymorphisms in expressed sequence tags dataset of Allium cepa L.., Online Journal of Bioinformatics . Volume 12(1):34-45, Radika V, Lakshmana Reddy D C, Archana B, Shewta and Aswath C., 2011, In silico microsatellite development in Arum lily (Zantedeschia aethiopica), Journal of Horticultural Sciences 26(1):37-40. Dhanya,Lakshmana Reddy D C, Veerae Gowda and Aswath C, 2013, The genetic relatedness analysis of male sterile and their maintainer lines of onion (Allium cepa L.) by using RAPD markers. Journal of Crop science and Biotechnology,.March volume, Springer publications. Kanupriya, M Shobana, C Vasugi, C Aswath, V Radhika, Laxman Reddy and M R Dinesh. 2012.Genetic relationship among papaya (Carica papaya) and wild papaya (Vasconcellea species) using RAPD and ISSR markers. Indian Journal of Agricultural Sciences 82 (4): 368–71. Kanupriya; P. Madhavi Latha; C. Aswatha; Laxman Reddy; B. Padmakara; C. Vasugic; M. R. Dineshc 2011, Cultivar Identification and Genetic Fingerprinting of Guava (Psidium guajava) Using Microsatellite Markers. International Journal of Fruit Science 11:184-196. Taylor and Francis, UK D.C.Lakshmana Reddy, H.H.Kumara swamy , Kanti meena and V.Dinesh Kumar,2009. “Host-pathogen bargaining via molecular signals- A brief review”, J.Oilseeds Res., Vol 26 (Special issue) pp181-8183. Kanti Meena , H.H.Kumara swamy, D.C.Lakshmana Reddy, V.Dinesh Kumar and D.K.Meena, 2009, Gamma linolenic acid (GLA) enhancement through genetic engineering in oilseeds crops: a brief review . J.Oilseeds Res., Vol 26 (Special issue) pp185-188. .H.Kumara swamy, V.Dinesh Kumar D.C.Lakshmana Reddy, Kanti meena , K.Sai kumar, V.Srikanth and B.Madhu, 2009, Single cell oil: can it be supplementary to plant oils in meeting the growing demand? J.Oilseeds Res., Vol 26 (Special issue) pp217-220, 2009. . List of papers presented in conferences • Delivered a Oral presentation on “ Bioinformatics work at IIHR” at National seminar on “Hortinformatics-National consultative meet on Bioinformatics in Horticulture” held at IISR, Calicut held on 11th-12th October 2010. • Delivered a Oral Presentation on “Molecular markers for breeding fungal resistance in horticultural crops” at National seminar on “National symposium on Molecular approaches for management of fungal diseases of crop plants” held at I.I.H.R, Bangalore held on 27-30 December 2010. Delivered a lead presentation on " Role of molecular markers in diseases resistance breeding in horticultural crops" at 10th national symposium on " biotechnological approaches for plant protection: constraints and opportunities held at ICAR research complex for Goa, from Jan 27-29, 2013. Delivered a lead presentation on "Genomics in Horticulture: Present status and future needs" at Global conference on Horticulture for food, Nutrition, and livelihood options held at Bhubaneswar, India from May28-31, 2012. Poster presentation on DNA based Diagnostics for identification of citrus rootstocks cv Rangapur lime, Rough lemon and Pseudo lemon at International conference on plant biotechnology for food security : New Frontiers held at NAAS center, Pusa, NewDelhi from Feb 21-24, 2012 Delivered a invited lecturer on " Development of SCAR markers for specific traits at NRC banana , Trichy during workshop on Theory and Practices on Advances in gene identification and marker development from 1-10 Oct 2012 Poster presentation on "Identification of molecular markers linked to resistance against bacterial wilt ( Ralstonia solanacearum) in eggplant" at 7th International symposium on In vitro culture and horticultural breeding “Biotechnological advances in In vitro Horticultural breeding” at Ghent, Belgium, Europe from 18/09/2011 to 22/09/2011 Book Chapters C.Aswath and Lakshmana Reddy D C., Gerbera and Carnation: Molecular markers and Markers assisted selection: Advances in Horticultural Biotechnology, Vol-IV: Vegetables,ornamentals and tuber crops. Edited by H.P.Singh., V. A. Parthasarthy and Nirmal Babu., Published by Westville Publishing House. New Delhi. C.Aswath and Lakshmana Reddy D C., Gladiolus: Molecular markers and Markers assisted selection: Advances in Horticultural Biotechnology, Vol-IV: Vegetables, ornamentals and tuber crops. Edited by H.P.Singh., V. A. Parthasarthy and Nirmal Babu., Published by Westville Publishing House. New Delhi. C.Aswath and Lakshmana Reddy D C., Lilium: Molecular markers and Markers assisted selection: Advances in Horticultural Biotechnology, Vol-IV: Vegetables, ornamentals and tuber crops. Edited by H.P.Singh., V. A. Parthasarthy and Nirmal Babu., Published by Westville Publishing House. New Delhi.
Selected as a Scientist (Biotechnology) through competitive examination for Agricultural research service scientist (biotechnology) recruitment conducted by ASRB, New Delhi (Topper in merit list) Backward classes and minorities (BCM) residential scholarship in B.Sc(Ag) and M.Sc (Ag), Government of Karnataka, India Patents under process/filed : 1. DNA Based Diagnostics for Identification of Citrus rootstock cultivars Rangpur Lime, Rough Lemon and Galgal( Indian patent) Technologies Developed: • SSR based assay Identification of F1 hybrid of Solanum melongena and Solanum Macroparon • SSR based Hybrid purity assessment assay for Arka Meghana • Multiplex PCR assay for identification citrus rootstocks Development of unique DNA barcodes: Eggplant Varieties- IIHR-3, IIHR-7, Arka Keshava, 2 BMG 1, SMS 6-6