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A SIMPLE SSR BASED ASSAY TO IDENTIFY INTERSPECIFIC HYBRIDS BETWEEN SOLANUM MELONGENA LAND SOLANUM MACROCARPON L

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Technologies

 

testing of genetic purity of eggplant hybrid (IIHR-3 X S. macrocarpon L) with two codominant SSR markers- emf01C03 (Left Side) and emh02E08( Right Side) , Lanes:P1 is S.melongena Variety IIHR-3 and P2 is S.Macrocarpon L, 1 -10 are F1 Hybrids, M-DNA

 

Technology Description

  • Traditionally hybrid purity of F1 assessed through grow out test (GOT) is time consuming and labour intensive.
  • There is need of fast and reliable alternative method for hybrid purity assessment.
  • By using SSR, developed a simple SSR-PCR based assay to identify interspecific hybrids between S. melongena and S. macrocarpon.

 

Products and By- products

  • SSR based assay Kit

 

Technology Benefits

  • It will identify selfed/mechanical mixtures or correctly crossed hybrids or progeny
  • works on codominant nature

 

Target segment /End User Profile

  • Seed Companies,
  • Brinjal breeders,
  • To solve copyrights, trademarks,
  • To identify admixtures, mis labeled seeds or mechanical mixtures in seed lots.

 

Market potential

  • This study clearly revealed the efficacy of genomic-SSRs in genetic purity of eggplant hybrids; can identify the admixture of parents in the hybrid seed lot.
  • In addition, this testing would save the cost of hybrid seed storage for a whole season and the cost incurred on GOT.
  • This purity test can be employed in seed companies for accurate and reliable detection of off-types in commercial eggplant seeds for ensuring the supply of good quality seeds to the market.
 
 
Service:
  • Genetic hybrid assay kits can be developed for seed company’s hybrids and testing
 
Investments required
  • 5 lacs
 
Expected returns ratio
  • They can use in addition or in lieu of GOT, Therefore can save lot of money.