बीज की गुणवत्ता-परीक्षण के लिए जैव-मार्कर

Enzyme activity- Dehydrogenase activity- viability test through  TZ test

 

 

Protein profiles- loss of  protein subunits  with advancement in ageing and loss of viability

      100    100     37    22          0     0 %        Germination %

 

 

DNA integrity-. Loss of DNA integrity and shearing of DNA with advancement in ageing and loss of viability

Note; M-marker, D-Days  after accelerated ageing

                F          3D         6D      9 D      12D       15D            M  

 

 

Genetic purity testing- Biochemical and Molecular markers

Proteins- storage proteins

Dominant markers

Staining sensitive

Compact profile –difficult to identify though markers present

 

    F              H            M               F              H            M

 

 

Isozymes- Peroxidases

Co-dominant

Non-toxic

Staining sensitive and stage specific

Markers from both the parent

          F         H          M

 

 

DNA markers- SSR markers

Co-dominant

Reliable and stable

Markers from both the parent

Note: F- female parent  H- hybrid  M-male parent

Crop: Chillies