I like to work with plant viruses, I use the techniques of TEM, ELISA, DIBA, Probes, and sequence analysis. I try to over-express the coat proteins and make immuno probes for them. I also like to manage field problems caused by plant viruses and visualize the data I worked from 1993 to 2003 at The Virology Lab, IIHR, Bangalore and at Central Horticultural Experiment Station (CHES) Chettalli, Kodagu, Karnataka from 2003 to 2007. from 2008 I am at The Virology Lab, IIHR, Bangalore . 1. Principal Investigator for the ICAR Adhoc Scheme "Development of Immunodiagnostic Kit for Papaya Ring Spot Virus" (from 1.10.1997 to 30.9.2000 with a budget of Rs 21,24,214.00) 2. Principal Investigator of the Indian Council of Agricultural Research, National Agricultural Technology Project, Competitive Grant Project (ICAR/NATP-CGP) “Molecular Beacon Based Diagnosis of Banana Bunchy Top Virus and Banana Bract Mosaic Virus”.(from 1.4.2001 to 31.3.2002 with a budget of Rs 37,12,478.00) Co- principal Investigator 1.National Facility for Virus Diagnosis and Quality Control an ICAR & DBT a nationwide program for testing planting materials for plant viruses especially of fruit crops. 2. Development of Transgenic Papaya Resistant to Papaya Ring Spot Virus, an Indian Council of Agricultural Research, National Agricultural Technology Project, Competitive Grant Project (ICAR/NATP-CGP) 3.Diagnostic approaches and development of management strategies for rejuvenation of Coorg mandarin, a Department of Horticulture, Government of Karnataka funded project.
Scientific:
1. Duleep Kumar Samuel., Mohan.K,S.,Jagadish Chandra, K and Leela Shajiram (1999) Detection of Bunchy Top Virus in Micropropagated Plants Using a PCR generated DNA Probe. Pest Management in Horticultural Ecosystems, 5: (1) 32-37.
2. Duleep Kumar Samuel, Dheeraj Shetty, Ravishankar,H., Gopalakrishna Pillai, K., Sakthivel,T., Karunakaran,G., Raghupathi,H.B., and Sudhir Kumar (2007). Molecular Detection and Analysis of Asian Strain Specific LAS Fragment of Huanglongbing Bacterium (Candidatus Liberibacter asiaticus) Vectoried by Diaphorina citri Kuwayama (Hemiptera:Psyllidae) Associated with Decline of Coorg Mandarin (Citrus reticulate Blanco) Pest Management in Horticultural Ecosystems, 13: (2) 91-101.
3. Gopalakrishna Pillai,K., Duleep Kumar Samuel,L.,and M.Krishna Reddy. (2008). Studies on the transmission of citrus greening bactgerium, Candidatus Liberibacter asiaticus, by the vector citrus psylla, Diaphoina citri Kuwayama in Coorg Mandarin. Entomon.,32(4):291-295. Book Chapters: 1.L.Duleep Kumar Samuel and V,Mariappan (1994). Integrated Management of Chilli Mosaic Virus and its Vectors. In Crop Diseases - Innovative Techniques and Management (Ed) K.Sivapragasam and K.Seetharaman.Chapter 49. Kalyani Publishers, Ludhiana, India pp 361-374. 2.K.Jagadish Chandra and L. Duleep Kumar Samuel (1999). Viral and Phytoplasmal Diseases of Papaya (Carica papaya.L) In India. In Diseases of Horticultural Crops : Fruits. (Ed) L.R. Verma and R.C.Sharma. Chapter 22. Indus Publishing Co, New Delhi p 724. Technical Bulletins: 1. Improved Technology for Coorg mandarin Production by H. Ravishankar, T. Sakthival, D.K.Samuel , H.B. Raghupathy, G.K. Pillai and G. Karunakaran. Technical Bulletin No. 31 published by the Indian Institute of Horticultural Research, Bangalore 2. Rambutan : An under utilized fruit crop for tropical humid climates by H. Ravishankar, T. Sakthival, D.K.Samuel and K.Shivaramu. . Technical Bulletin No. 32 published by the Indian Institute of Horticultural Research, Bangalore Extension folders: 1. After care of disease free Coorg mandarin budded plants by H. Ravishankar, H.B. Raghupathy, G.K. Pillai, T. Sakthival, D. K. Samuel and G. Karunakaran. (English) . Extension folderNo. 75 published by the Indian Institute of Horticultural Research, Bangalore 2. After care of disease free Coorg mandarin budded plants by H. Ravishankar, H.B. Raghupathy, G.K. Pillai, T. Sakthival, D.K.Samuel and G. Karunakaran. (Kannada) Extension folder No. 76 published by the Indian Institute of Horticultural Research, Bangalore.
1. Duleep Kumar Samuel., Mohan.K,S.,Jagadish Chandra, K and Leela Shajiram (1999) Detection of Bunchy Top Virus in Micropropagated Plants Using a PCR generated DNA Probe Pest Management in Horticultural Ecosystems, 5: (1) 32-37. Awarded the Vikram Govind Prasad Award for Best Paper published during 1996-2000 in the Journal Pest Management in Horticultural Ecosystems by the Association for Advancement of Management in Horticultural Ecosystems.
2. Duleep Kumar Samuel, Dheeraj Shetty, Ravishankar,H., Gopalakrishna Pillai, K., Sakthivel,T., Karunakaran,G., Raghupathi,H.B., and Sudhir Kumar (2007). Molecular Detection and Analysis of Asian Strain Specific LAS Fragment of Huanglongbing Bacterium (Candidatus Liberibacter asiaticus) Vectoried by Diaphorina citri Kuwayama (Hemiptera:Psyllidae) Associated with Decline of Coorg Mandarin (Citrus reticulate Blanco) Pest Management in Horticultural Ecosystems, 13: (2) 91-101. Awarded the Vikram Govind Prasad Award for Best Paper published during 2005 - 2010 in the Journal Pest Management in Horticultural Ecosystems by the Association for Advancement of Management in Horticultural Ecosystems. (Second Time)
3. Dheeraj,C., Sathiya priya, N, Ravishankar,H., Raghupathi,H.B., Pillai,G.K., Sakthivel,T., Karunakaran, G and Samuel.D.K (2006) Awarded best poster presentation for “Evaluating the Role of Viral and Allied Pathogens in Coorg mandarin Decline”. In National Symposium on Citriculture: A Road Map, held at ICAR Research Complex for NEH Region at Umiam, Meghalaya from 22-24th Feb., 2006.
4. Karunakaran, G., H.Ravishankar,H., Samuel, D.K., Sathiyapriya, N. (2006) Awarded best poster presentation for “Standardization of Shoot Tip Protocols for Coorg Mandarin. In National Symposium on Citriculture: A Road Map at ICAR Research Complex for North East Hill Region, Umiam, Shillong, Meghalaya from 22 - 24 February 2006.
5. Samuel, D.K. Dheeraj,C., Ravishankar,H., Pillai,G.K., Sakthivel,T., Karunakaran, G., Raghupathi,H.B and Sudhir Kumar (2007). Awarded certificate for best oral presentation for the paper entitled “Molecular detection and analysis of Huanglongbing bacterium associated with Coorg mandarin Decline”. In: Third National symposium on Plant Protection in Horticulture Emerging Trends and Challenges held at IIHR, Bangalore on March 7-9, 2007
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