Enzyme activity- Dehydrogenase activity- viability test through TZ test
Protein profiles- loss of protein subunits with advancement in ageing and loss of viability
100 100 37 22 0 0 % Germination %
DNA integrity-. Loss of DNA integrity and shearing of DNA with advancement in ageing and loss of viability
Note; M-marker, D-Days after accelerated ageing
F 3D 6D 9 D 12D 15D M
Genetic purity testing- Biochemical and Molecular markers
Proteins- storage proteins
Dominant markers
Staining sensitive
Compact profile –difficult to identify though markers present
F H M F H M
Isozymes- Peroxidases
Co-dominant
Non-toxic
Staining sensitive and stage specific
Markers from both the parent
F H M
DNA markers- SSR markers
Co-dominant
Reliable and stable
Markers from both the parent
Note: F- female parent H- hybrid M-male parent
Crop: Chillies
