Protocol for large scale propagation of of selected pre-release hybrid of Hippeastrum using bulb scale explants (both single and twin scale )has been standardized.
Proliferation of shoots could be achieved by subculturing the bulblets generated in the same medium. There was no need of a rooting medium as the same proliferating medium could induce root formation.
Thus the tissue culture protocol developed would facilitate the breeding programmes aimed at developing improved varieties of Hippeastrum. Using 4 bulbs, 200 micropropagated shoots were obtained in 6 months time. About 100 plants were hardened.