Country’s first sporeless mutant of Pleurotus was produced through UV radiation mutation. This mutant was produced to counter the respiratory allergy caused due to inhalation of spores in the commercial farms. The sporeless mutant does not produce any spore as compared to the parent P. florida which produced 9478444 spores/ml This character has been stable since the past 15 years. The yield of this mutant had increased by 38%.